5/30/2023 0 Comments Western blot test![]() Envelope (gp120,gp160,gp41), GAG (p17,p24&p55) and POL(p51, p31 & p66).įor a sample to be HIV Positive, a Control band and at least 2 HIV envelope bands along with any 1 GAG or 1 POL band shall be present on the strip. The results are interpreted as Negative, Positive, or Indeterminate based on the presence of bands on the strip at the corresponding HIV Antigen. ![]() The test is run on strips blotted with HIV viral antigens, and the blue color bands appear on the strip after the test is completed due to the use of Alkaline phosphatase enzyme and BCIP –NBT substrate in the process. HIV Testing is performed using screening test Rapid /ELISA and samples found reactive in these tests are confirmed for their status by Western blot. The Western Blot test is the confirmatory test to detect antibodies to HIV (Human immunodeficiency virus), the causative agent of AIDS (Acquired Immunodeficiency Syndrome). Western blotting is used for the confirmatory medical diagnosis of various infectious diseases and, to name a few, HIV, bovine spongiform encephalopathy (BSE), hepatitis C (HCV), syphilis, and autoimmune disorders.Īs per WHO guidelines, this technique has been widely used as a more specific & supplemental assay on samples found initially reactive using ELISA and other screening tests for HIV. Western blot is a technique used for protein detection from a mixture of proteins and has its application in immunology, molecular biology, biochemistry, and cell biology fields. ![]() Significance Of Malaria Antigens Over Antibodies.
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